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11.22 学术活动
2010-11-21 11:20   发布范围:公开

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据药理学教研室与药剂学教研室共同学术会议安排,1122日早800由张蓉博士为大家做学术报告,主要阐述模拟微重力条件对骨质形成的影响,英文名称为Modeled Microgravity Inhibits Osteogenic Differentiation of Human Mesenchymal Stem Cells andIncreases Adipogenesis. 热烈欢迎感兴趣的同学和老师参加并讨论。

本文采用一种细胞培养的方式模拟微重力条件,提供了一种研究微重力的新方法。到底如何实现?敬请关注。

 

文章摘要如下:

 

Space flight-induced bone loss has been attributed to a decrease in osteoblast function, without a significant change in bone resorption. To determine the effect of microgravity (MG) on bone, we used the Rotary Cell Culture System [developed by the National Aeronautics and Space Administration (NASA)] to model MG. Cultured mouse calvariae demonstrated a 3-fold decrease in alkaline phosphatase (ALP) activity and failed to mineralize after 7 d of MG. ALP and osteocalcin gene expression were also decreased. To determine the effects of MG on osteoblastogenesis, we cultured human mesenchymal stem cells (hMSC) on plastic microcarriers, and osteogenic differentiation was induced immediately before the initiation of modeled MG. A marked suppression of hMSC differentiation into osteoblasts was observed because the cells failed to express ALP, collagen 1, and osteonectin. The expression of runt-related transcription factor 2 was also inhibited. Interestingly, we found that peroxisome proliferatoractivated receptor _ (PPAR_2), which is known to be important for adipocyte differentiation, adipsin, leptin, and glucose transporter-4 are highly expressed in response to MG. These changes were not corrected after 35 d of readaptation to normal gravity. In addition, MG decreased ERK- and increased p38-phosphorylation. These pathways are known to regulate the activity of runt-related transcription factor 2 and PPAR_2, respectively. Taken together, our findings indicate that modeled MG inhibits the osteoblastic differentiation of hMSC and induces the development of an adipocytic lineage phenotype. This work will increase understanding and aid in  the prevention of bone loss, not only inMGbut also potentially in age-and disuse-related osteoporosis. (Endocrinology 145: 2421–2432, 2004)

附件【Modeled Microgravity Inhibits Osteogenic Differentiation of Human Mesenchymal Stem Cells and Increases Adipogenesis.pdf已下载
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