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10.25学术会议
2010-10-23 17:20   发布范围:公开

 

Current Cover据药理学教研室与药剂学教研室共同学术会议安排,10月25日早8:00由张邦乐教授为大家做学术报告,主要讲解一种纳米材料的在体基因递送研究结果,英文名称为In vivo gene delivery by cationic tetraamino fullerene. 热烈欢迎感兴趣的同学和老师参加并讨论。

纳米技术在医药研究领域的应用已经取得巨大成功,前人的研究发现,水溶性呋仑碳在体外基因递送方面的潜能明显优于阳离子脂质体。然而,其在体研究却未曾有人报到,其效果究竟如何,请到时听张教授分解。

 

文章摘要如下:

 

Application of nanotechnology to medical biology has brought remarkable success. Water-soluble fullerenes are molecules with great potential for biological use because they can endow unique characteristics of amphipathic property and form a self-assembled structure by chemical modification. Effective gene delivery in vitro with tetra(piperazino)fullerene epoxide (TPFE) and its superiority to Lipofectin have been described in a previous report. For this study, we evaluated the efficacy of in vivo gene delivery by TPFE. Delivery of enhanced green fluorescent protein gene (EGFP) by TPFE on pregnant female ICR mice showed distinct organ selectivity compared with Lipofectin; moreover, higher gene expression by TPFE was found in liver and spleen, but not in the lung. No acute toxicity of TPFE was found for the liver and kidney, although Lipofectin significantly increased liver enzymes and blood urea nitrogen. In fetal tissues, neither TPFE nor Lipofectin induced EGFP gene expression. Delivery of insulin 2 gene to female C57/BL6 mice increased plasma insulin levels and reduced blood glucose concentrations, indicating the potential of TPFE-based gene delivery for clinical application. In conclusion, this study demonstrated effective gene delivery in vivo for the first time using a water-soluble fullerene.

 

附件【2010PNASIn_vivo_gene_delivery_by_cationic_tetraamino_fullerene.pdf已下载
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